Previous page Carlos PARRAS Principal Investigator, PhD, CR1, INSERM Team “Genetics and physiopathology of epilepsy” http://carlosparras8.wix.com/website
BiographyCarlos Parras is expert in molecular neurobiology and genetics, and principal investigator (CR1 at INSERM from 2008). He did his PhD in Neurobiology using Drosophila as genetic model at the Center of Molecular Biology Severo Ochoa of the University Autonoma of Madrid (1997). He performed a postdoctoral training working in F Guillemot´s lab with mouse genetic models studying neural stem cells commitment to different neural subtypes in Strasbourg (IGBMC, 1998-2003) and London (NIMR, 2003-2006). He obtained an AVENIR grant to develop his research group in the Salpêtrière Hospital (Inserm U711, 2006-2011), and he joined the group of JL Thomas & B Zalc (2009) focusing on transcriptional regulation of oligodendrogenesis, using genetically modified and focal de/remyelination mouse models together with analysis of post-mortem brain samples from patients with Multiple Sclerosis. He demonstrated the role of Ascl1, a key neurogenic transcription factor, in oligodendrocyte specification and differentiation during myelination and remyelination. His recent research, characterizing Ascl1/Olig2 common gene targets, showed important role in oligodendrocyte differentiation during myelination and remyelination of CHD7 & CHD8 chromatin remodelers, whose haploinsufficiency in humans lead to CHARGE syndrome and Autism Spectrum Disorder, respective. He received the Prize Marie-Ange Bouvet Labruyère given by the Fondation de France in Feb 2018 for this work. His lab has established genome-wide approaches to study the transcription regulation of brain cells, with a focus in oligodendroglia as model system, to address the cooperation between transcription factors (such as Ascl1, Olig2 & Sox10) and chromatin remodelers (Chd7 & Chd8) in transcription regulation and cell fates (generation, proliferation, survival, and differentiation) during normal brain development and pathology (including Autisms, pre-term birth, and Multiple Sclerosis). In 2019, he joint the Brain development team led by B Hassan, and has contributed to projects in mouse brain development and human cultures mimicking corticogenesis from induced pluripotent stem cells (iPSCs), particularly in the role of human APP during cortical neurogenesis. His group has optimised protocols and analyses for genome-wide studies at the transcriptomic (bulk and single cell RNA-seq) but also chromatin levels (ChIP-seq for transcription regulators and regulatory histone marks, and ATAC-seq).
Research workThe current projects of his group include:
- 1. Transcription regulation of gliogenesis: a play between transcription factors and chromatin remodelers
- 2. Characterization of the molecular mechanisms of Tns3 function in oligodendroglia
- 3. Pharmacogenomics identification of small bioactive molecules for promoting oligodendrogenesis in a model of neonatal brain injury (project funded by the ANR 2017-2022)
- 4. Molecular mechanisms of Ascl1 function in oligodendrogenesis versus neurogenesis
- 5. Assessing novel FDA-approved drugs with validated in vitro oligodendrogenic activity in preclinical mouse models of multiple sclerosis (proposed for funding to the ARSEP in 2021)
- 6. The dose requirement CHD8 & Chd7 chromatin remodelers in the diverse genetic programs of both oligodendrocyte progenitors and neuronal progenitors
- Marie C, Clavairoly A, Frah M, Hmidan H, Yan J, Zhao C, Van Steenwinckel J, Daveau R, Zalc B, Hassan B, Thomas JL, Gressens P, Ravassard P, Moszer I, Martin DM, Lu QR, and Parras C. Oligodendrocyte precursor survival and differentiation requires chromatin remodeling by Chd7 and Chd8. PNAS. 17 August.
- He D, Marie C, Zhao C, Wang J, Deng Y, Kim B, Clavairoly A, Frah M, Wang H, He X, Hmidan H, Jones BV, Witte D, Zalc B, Zhou X, Choo DI, Martin DM, Parras C*, Lu QR* (2016). Chd7 cooperates with Sox10 and regulates the onset of CNS myelination and remyelination Nat. Neurosci. 29 Feb 2016 AOP. * Corresponding authors.
- Nakatani H, Martin EM, Hassani H, Clavairoly A, Maire CL, Viadieu A, Kerninon C, Delmasure A, Frah M, Weber M, Nakafuku M, Zalc B, Thomas JL, Guillemot F, Nait-Oumesmar B, Parras C (2013). Ascl1/Mash1 promotes postnatal oligodendrogenesis and remyelination in the mouse cortex. JNeurosci. 33 (23): 9752-9768
- Parras, CM, Galli, R, Britz, O, Soares, S, Galichet, C, Battiste, J, Johnson, J E, Nakafuku, M, Vescovi, A and Guillemot, F. (2004). Mash1 specifies neurons and oligodendrocytes in the postnatal brain. EMBO J 23, 4495-505.
- Parras CM, Schuurmans C, Scardigli R, Kim J, Anderson DJ and Guillemot F (2002). Divergent functions of the proneural genes Mash1 and Ngn2 in the specification of neuronal subtype identity. Genes Dev. 16, 324-338.