ACTIVITIES
• Production and caring of transgenic and mutant lines
• PCR genotyping
• Transgenesis: Tol2/CrispR/Talen
• Fluorescent In situ hybridization (FISH)
• Fluorescent immunohistochemistry on embryos / larvae in toto or on slice
• Confocal, bi-photon and spinning disk imaging
• Immuno-reactive area quantification on ImageJ
• Behavioral analysis, results interpretation on mutant and drug screening
• Sperm Freezing
• Electron microscopy on embryos and larvae (1-3 days)
• Calcium imaging and optogenetics on wild type animals and transgenic lines
• New lines for sensors and actuators of neuronal activity in vivo
EQUIPMENT
• Quarantine: 100 aquariums
• Housing area: 900 aquariums (about 20,000 adults)
• Daily artemia production
• Automated feeding of adults with a robot
• More than 500 transgenic lines
• In vitro fertilisation
• Health follow up program (PCR analysis and histology)
• 6 injectors optimized for mutagenesis, transgenesis and genetic knock down
• High-throughput crossing with the iSpawn for high scale eggs production