CELIS–iPS Stem cell core facility

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Human induced-pluripotent stem (iPS) cells are produced in order to model in a culture dish degenerative disorders of the brain and the spinal cord. This is to better investigate the molecular and cellular mechanisms underlying these disorders and to develop a screening approach for drugs of therapeutical interest. The specific goals of the platform are three-fold:
1– to generate and characterize iPS cells through genetic reprogramming of skin cells from patients and healthy volunteers;
2– to correct or introduce mutations of interest using appropriate genetic tools;
3– to create a repository of disease-specific iPS cells. This activity is part of the Institut du Cerveau – ICM’s cell culture facility and is supported by the IHU-A-Institut du Cerveau – ICM.

ACTIVITIES

• Services:
Cellular reprogramming, molecular and functional characterization of iPS cells, maintenance and storage of iPS cells and in future, genetic engineering of iPS cells
• Provision of equipment for autonomous users
• Scientific and technical advice to users
• Optimization and improvement of technical procedures

EQUIPMENT

• Level II Biocontainement box with two workstations each containing:
– Lynx Dynascope under class II biological safety cabinet
– CO2 incubator
• Inverted microscope with phase contrast optics
• Two inverted fluorescence microscopes with cooled camera to acquire digitalized images are accessible in a dark room next to the CELIS-iPS core facility
• Nucleofector 4D for electroporation

TEAM

Delphine Bohl —Scientific manager
Patrick Pierre Michel —Scientific manager
Stéphanie Bigou —Operational manager, engineer

CONTACT

stephanie.bigou@icm-institute.org