CELIS-E-PHYS : electrophysiology core facility

CELIS-ePhys is an unique core facility providing high quality contract research services to academics and pharmaceutical industry requiring in vitro and ex vivo electrophysiology technique for successful completion of neurosciences studies. The facility is equipped for both patch-clamp and field potential recordings. The facility is involved in many different research project as diverse as the studies of channelopathies, human iPSC derived neurons and neural plasticity. CELIS-ePhyS is part of the Institut du Cerveau – ICM core facility network(sequencing, vectorology, cell culture, iPSC…) which facilitates translational research projects. This activity is supported by the IHU-A-Institut du Cerveau – ICM funding.

ACTIVITIES

• Generation of full set of data by expert staff (experimental design, data acquisition, analysis and interpretation)
• Access to the equipment for independent use• Preliminary experiments designed to assess project feasibility
• Consulting
• Training scientists to electrophysiology techniques
• Development of new assays on demand

EQUIPMENT

• Patch-clamp rig equipped with an inverted fluorescence microscope, amplifier and micromanipulator for patch-clamp recordings from cell culture
• Patch-clamp rig equipped with an upright fluorescence and optogenetic microscope, amplifier and micromanipulator for patch- clamp recordings from brain slice and zebrafish
• Multielectrode Array (MEA) system for multi- recordings of extracellular field potential
from brain slices and cell culture, including iPSC-derived cells (field evoked postynaptic potential (fPSP) and spontaneous spike)
• Fast pressurized system for recording of ligand-gated ion channels and fast drug application; a temperature control system• Electrode puller, microforge…

TEAM

Patrick Pierre Michel —Scientific manager
Carine Dalle —Operational manager & Research engineer
Charlotte Deleuze —Research engineer

CONTACT

celis-ephys@icm-institute.org