ACTIVITIES
• Modeling brain, spinal cord and skeletal muscle disorders with cell or tissue culture systems
• Molecular and cellular pharmacology; Medium throughput screening
• Conventional or automated fluorescent imaging; infrared fluorescence imaging
• High throughput fluorescence imaging; Functional imaging; Videomicroscopy
• Near infrared or chemiluminescence imaging for blot detection
• High performance electrochemiluminescence immunoassays
• Flow cytometry for cell analysis
• Electrophysiological characterization of ion channel dysfunction (CELIS-ePhyS)
• Production and characterization of iPS cell lines (CELIS-iPS)
EQUIPMENT
• 18 cell culture boxes (L2 confinement) with CO2 incubators and safety cabinets
• 2 automated workstations for high throughput imaging (Arrayscan XTi, CellInsight)
• Database and application server with remote PC workstations for post-hoc image analysis
• 5 inverted fluorescent microscopes (3 Zeiss Axio Observer7; 1 Nikon Ti Eclipse; 1 NikonTE 2000) for functional imaging, videomicroscopy or optical sectioning (ApoTome)
• Bright field and fluorescence stereomicroscopes
• Odyssey and ChemiDoc Touch imaging systems for blot detection
• MESO QuickPlex SQ 120 for multiplex immunoassays
• BD FACSVerse flow cytometer; automated cell counters (Tali, Countess)
• 2D and 4D Nucleofector systems
• Workstation for automated cell culture
• 1 box for the generation of iPS cell lines
• 1 box for manipulating radiochemicals
• Set-ups for electrophysiological recordings on isolated cells, zebrafish or brain slices
TEAM
Patrick-Pierre Michel — Scientific manager
Laetitia Strehl — Operational manager
David Akbar – Engineer, Cell culture imaging
Jérôme Garrigue – Engineer, Automated cell culture
Stéphanie Godard-Bauche – Engineer, Operational support
Carine Dalle —Research engineer, electrophysiology
Charlotte Deleuze – Research engineer,
Stéphanie Bigou — Engineer, IPS cell production