ACTIVITIES
• High-Throughput using Illumina system including Exome, RNAseq (including single cell, mRNAseq, total RNAseq, 3’RNAseq), MethylSeq (Capture or whole genome bisulfite), miRNAseq, Genome, chlPseq and custom capture panel.
• Real-time PCR: SybrGreen or probespecific technology, RNA quantification, Droplet Digital PCR, HRM
• Sequencing: medium-throughput using Miseq Illumina systems including amplicon sequencing, long-range, ultra-deep sequencing applications and Capture (DNA and RNA)
• Genotyping: animals (tissue)
• PCR products purification and analysis: PCR or sequence purification and quantitative analysis of DNA or RNA
EQUIPMENT
• 3 Real-time PCR (1×96 wells, 384 wells, 1536 wells)
• 11 PCR apparatus (Applied Biosystem, MJ Research, Eppendorf)
• 1 Miseq Illumina system
• 1 Bravo pipetting system
• 1 plateLoc system
• 2 Labchip GX
• 2 NextSeq Illumina System
• 1 Novaseq6000 Illumina System
• 1 TapeStation Agilent System
• 1 Bioruptor Diagenode System
TEAM
Stephanie Baulac (Scientific coordinator)
Yannick Marie (Operational coordinator)
Jérôme Garrigue (Animal genotyping)
Bruno Choisi (Animal Genotyping)
Delphine Bouteiller (Real-time PCR and ISO9001)
Emeline Mundwiller (Sequencing)
Claire Sophie Davoine (Sequencing )
Agnès Rastetter (Sequencing)
CERTIFICATION
The activities of iGenSeq platform have been certified since 2015 regarding the ISO9001 standard (out of equipments provision activity, and animal’s DNA & RNA genotyping), allowing to guarantee the traceability, the reliability and the rigour of the implemented procedures.